Parasitological and histopathological study of chronic trichomonas prostatitis aided by transcrectal ultrasound biopsies

The aim of this work was to detect the role of transrectal prostatic biopsy and the use of different stains in the diagnosis of chronic trichomonas prostatitis. Trichomoniasis vaginalis was diagnosed in 28% of 100 male patients with urethritis. The diagnosis was based on the detection of Trichomonas vaginalis trophozoites in urethral discharge by wet mount and Giemsa positive stain which was seen in 28 cases, while trophozoites were detected in the sediments of centrifuged urine of only 21 cases. Among 28 trichomonas positive urethritis patients, 14 cases were diagnosed clinically as chronic prostatitis. The histopathology in ten cases confirmed the diagnosis of trichomonas chronic prostatitis by demonstrating Trichomonas vaginalis trophozoites in the prostatic biopsies stained with both modified periodic acid Schiff and Giemsa stains. All patients were cured both clinically and parasitologically after treating them and their parents with metronidazol. In conclusion, better results were obtained by the use of easy available and cheap stains for the detection and identification of T. vaginalis in prostatic biopsies

Cryptosporidium parvum and microsporidial species in obstructive biliary diseases

Biliary and duodenal aspirates were collected from 60 patients to detect Cryptosporidium parvum [C. parvum] and microsporidial species in obstructive biliary diseases. Patients were classified into three groups: Group I, benign obstructive biliary diseases [30 cases]; Group II, malignant obstructive biliary diseases [30 cases] and Group III, ten normal persons served as controls. C. parvum oocysts were detected in higher percentage in malignant obstructive biliary diseases than benign obstructive biliary diseases, but no oocysts were detected in stool samples. Microspora was also detected in higher percentage in group II than group I. Concerning types of obstructive biliary diseases in benign type, C. parvum and microspora were detected with a higher percentage in calcular type than stricture or dyskinesia. In malignant obstructive group, a higher percentage of C. parvum oocysts was detected in carcinoma of biliary tree

Light and electron microscopic studies on skeletal muscle of mice infected with schistosoma mansoni

Skeletal muscles were collected from mice 12 weeks after being experimentally infected with Schistosoma mansoni [Sm]. Light microscopic [LM] examination revealed that some of the muscle fibers became disorganized, lost their transverse striation and sometimes contained abnormally centrally located chains of 4-6 nuclei. Moreover, groups of fat cells and inflammatory cells were present in between muscle fibers. Under the electron microscope [EM] the majority of muscle fibers suffered mild to severe ultrastructural changes. Mildly affected fibers showed only abrupt change in the direction of their myofibrils from longitudinal to oblique or even transverse. In severely affected muscle fibers the myofibrils were not only disoriented but also were severely disorganized, The Z lines were thickened and sarcoplasmic reticulum elements were abnormally dilated. Moreover, these severely affected fibers contained degenerated mitochondria and large secondary lysosomes. The ultrastructure of the blood capillaries was severely affected. The walls were thickened because of the increased thickness of both the endothelial cells and their basal laminae. There was also severe reduction of the capillary lumena, which were frequently traversed by intercommunicating endothelial-cell cytoplasmic processes. No granulomata were detected however, few degenerated ova were seen under the EM. The present results confirm at the EM level, for the first time, the reported skeletal muscle - myopathic changes in response to Sm infection